Transdermal drug delivery system constructed by polyhydroxyalkanoates and PAMAM dendrimers

doi:10.3969/j.issn.2095-4344.2014.52.013

Abstract

Abstract:

BACKGROUND: Polyhydroxyalkanoates (PHA) can be used as an in vitro drug delivery carrier for its characteristics of viscosity and ability of drug mixture and dispersion. PAMAM dendrimers have unique physical and chemical properties as a drug carrier.

OBJECTIVE: To mix PHA with PAMAM to build a transdermal drug delivery system for indissolvable drugs.

METHODS: Pseudomonas putida KT2442 was cultured in a culture medium containing NH4Cl at 30 ℃ for 72 hours, then 5 g/L octane acid was added in the culture system and cultured for 24 hours, and PHA compound was extracted with chloroform. PHA compound matrix (10 mg) and PHA compound matrix containing PAMAM and phenethylamine dendrimers (10 mg) were respectively mixed with 5 mg of sulfonamide derivative Tamsulosin to construct a transdermal delivery system. Meanwhile, an in vitro permeation device was established to study the promoting effect of compound matrices on drug penetration. X-ray diffraction analysis was used to analyze the atomic spatial distribution of Tamsulosin, PHA, PHA compound matrix-Tamsulosin, and PHA compound matrix containing PAMAM dendrimers-Tamsulosin.

RESULTS AND CONCLUSION: PHA and mixture of PHA and PAMAM dendrimers could promote the transdermal effect of indissolvable drugs as a transdermal drug matrix. In the same condition, the mixture of PHA and PAMAM dendrimers could satisfy the clinical blood drug concentration as a transdermal drug matrix. This mixture can make drug model crystallized well in a highly ordered manner.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: polyhydroxyalkanoates, administration, cutaneous, osmosis

CLC Number:

R318

Miao Wei-wei, Wang Zhi-xiong, Zhang Jun-li, Yuko Ohno. Transdermal drug delivery system constructed by polyhydroxyalkanoates and PAMAM dendrimers[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(52): 8433-8437.

Figures/Tables 2

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